Proteomics and Metabolomics, a part of system biology, is foundational for a full understanding of a biological system including a metabolic process or disease state, and has received considerable attention since the sequencing of the human genome. Oxidative/nitrosative damage by reactive radical species appears to be central to the pathogenesis of many human diseases including cancer and is central to a broad range of biotic and abiotic stress and physiological responses in microorganisms, plants and other animals. Specific modified proteins or metabolites are generated following stressing insults and accumulate in different degenerated tissues and fluids, determining altered organ functionalities. Mass spectrometry based technologies have been propitious to the development of molecular medicine, especially in the discovery of diagnostic biomarkers of oxidative and nitrosative stress enabling early detection of diseases such as lung cancer from these biological fluids. Although Redox Proteomics and Metabolomics is currently a nascent field of research, the opportunities for mass spectrometry based biomarker discovery involved in disease strictly linked to oxidative/nitrosative stress are clear and compelling. Clinical research also hopes to benefit from proteomics and metabolomics by both the identification of new drug targets and the development of new diagnostic markers Nature (422, 115-116; 2003).
Laboratory for Proteomics and Metabolomics analysis at the Redox Biology Center (RBC), Department of Biochemistry, UNL supports Faculties, scientists, clinicians and students within the RBC, University of Nebraska Lincoln, UNMC and other universities and industries. The Core provides all the tools of modern functional proteomics and metabolomics. The facility is equipped with chromatography and mass spectrometry based state-of-the-art technologies for proteomics and metabolomics (Clinical and non clinical), personalized experimental design consultation and comprehensive, individualized bioinformatics support.
We are interested in collaborations with faculties/ scientists in studying systems of biomedical importance such as clinical proteomics and metabolomics, biomarker discovery and validation, proteomic and metabolomics of plant/microbial and from other biological origin. Please contact Jiri Adamec, Core Director, regarding services provided.
All services provided are commonly used for basic biological research from biomarker discovery to target validation assay and pharmacokinetic studies.